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Wolfgang Kroutil

Lucas Hammerer: Regioselective fatty acid hydroxylation

Closed projects
Horst Lechner: Transformation of non natural substrates by norcoclaurine synthase
Elisabeth Eger: Tuning a Mn(IIII)-dependent alkene converting enzyme for biocatalytic applications

Further Information
Curriculum Vitae (47 kB)
Publication List (external Link)
Collaborators (46 kB)
Grants (55 kB)


Wolfgang Kroutil
Institute of Chemistry - Organic & Bioorganic Chemistry
Karl Franzens Universitšt Graz
Heinrichstrasse 28
8010 Graz

e-Mail: wolfgang.kroutil@uni-graz.at
phone: +43 316 380 5350
fax: +43 316 380 9840
web: http://biocatalysis.uni-graz.at

Tuning enzyme backbones for oxygen and metal dependent oxidations

W. Kroutilís research is dedicated to establishing enzymatic / biocatalytic methods for(asymmetric) organic synthesis. These aims are especially relevant for cases where traditional (chemical) methodologies are lacking or perform inefficiently or non-selectively. The main objectives are

(1) Redox reactions employing alcohol dehydrogenases, especially asymmetric reduction of ketones and !-chiral aldehydes, chemo- and regio-selective oxidation of prim- and sec-alcohols;

(2) Preparation of !-chiral primary amines employing "-transaminases;

(3) formation of C-C bonds;

(4) design of artificial concurrent enzyme cascades;

(5) Recycling of cofactors and co-substrates, redox-neutral redox-cascades;

(6)combination of enzyme catalysis with metal- and organo-catalysis;

(7) deracemisation concepts.

Laboratory know-how and infrastructure

Biocatalysis: testing of non-natural substrates, growth of bacteria, fungi, yeasts and extremophiles; shake flask experiments, biocatalytic transformations using (fermenting or resting) whole-cells or isolated enzymes, biotransformation upscaling up to 100 g substrate, enzyme reactions in organic solvents, fermentation (up to 10 L), enzyme purification, characterization and immobilization, selectivity-enhancement, modeling of kinetics.

Molecular biology: PCR, DNA mutagenesis, preparing DNA and cDNA libraries. Screening of libraries.

Biochemistry: Purification of enzymes.

Organic synthesis: Asymmetric synthesis of bioactive compounds using biocatalytic methods, experience in the synthesis of pheromones, vitamins, antibiotics, flavour- and fragrance compounds, pharmaceuticals.

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