Adaptation of enzymes for the transformation of non-natural substrates
(1) Redox reactions employing alcohol dehydrogenases, especially asymmetric reduction of ketones and !-chiral aldehydes, chemo- and regio-selective oxidation of prim- and sec-alcohols;
(2) Preparation of !-chiral primary amines employing "-transaminases;
(3) formation of C-C bonds;
(4) design of artificial concurrent enzyme cascades;
(5) Recycling of cofactors and co-substrates, redox-neutral redox-cascades;
(6)combination of enzyme catalysis with metal- and organo-catalysis;
(7) deracemisation concepts.
Laboratory know-how and infrastructure
Molecular biology: PCR, DNA mutagenesis, preparing DNA and cDNA libraries. Screening of libraries. Enzyme expression in E. coli and Pichia pastoris.
Biochemistry: Purification of enzymes. Organic synthesis: Asymmetric synthesis of bioactive compounds using biocatalytic methods, experience in the synthesis of pheromones, vitamins, antibiotics, flavour- and fragrance compounds, pharmaceuticals.
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