Function and Subcellular Localization of Aldehyde Dehydrogenase-2
B. Mayer is interested in the molecular pharmacology of signal transduction with special focus on the NO/cGMP system in the cardiovascular system. One major area of research in the past decade has been the function of NO synthases, in particular the role of the pterin cofactor tetrahydrobiopterin. With respect to the molecular actions of NO, he has studied the biological chemistry of physiologically and pathophysiologically relevant reactions such as S-nitrosation and protein tyrosine nitration. In the field of drug research, Dr. Mayer has studied the mechanism of action of NO synthase and soluble guanylyl cyclase inhibitors. For several years he has been working on the bioactivation of organic nitrates, in particular the role of aldehyde dehydrogenases and related enzymes, and the role of metabolic lipases in the cardiovascular system.
Laboratory know-how and infrastructureThe laboratory is well equipped for standard experimental work on protein purification and the biochemical/biophysical characterization of enzymes. For enzyme kinetic studies, a stopped-flow machine with a rapid scanning device and UV/fluorescence detection (Applied Photophysics) is available. In addition, standard equipment for molecular and cell biology as well as analytical biochemistry is available. The pharmacological equipment includes organ baths for smooth muscle relaxation studies and two Langendorff set-ups for perfusion of isolated hearts (mouse, rat, guinea pig), one with simultaneous measurement of intracellular Ca2+.